Application
WB analysis of extracts from CV-1 cells treated with forsklin. Lane 1.) Using antibody and no peptide. Lane 2.) Using antibody preincubated with peptide.Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LKB1 (phospho-Ser428) antibody| Product Name | LKB1 (phospho Ser428) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to LKB1 (phospho Ser428)LKB1, a member of the serine/threonine kinase family, is a tumor suppressor. Physically associates with p53, it regulates specific p53 dependent apoptosis pathways. LKB1 protein is present in both the cytoplasm and nucleus of living cells and translocates to mitochondria during apoptosis. Restoring LKB1 activity into cancer cell lines defective for its expression results in a G1 cell cycle arrest.Mutations in LKB1 gene are associated with Peutz Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. LKB1 functions as a master upstream protein kinase, regulating AMPK related kinases as well as AMPK. In vitro, LKB1 has been observed to directly phosphorylate threonine 172 of AMPKa, an event that is correlated with activation of this kinase. LKB1 has also been found to play a role in chromatin remodeling, cell cycle arrest, Wnt signaling, cell polarity, and energy metabolism. |
| Immunogen | Synthetic peptide - Human LKB1 around the phosphorylation site of serine 428 (R-L-SP-A-C) |
| Modification | p-Ser428 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| For ELISA: Use at a dilution of 1:500-1:1,000. For IHC-P: Use at a dilution of 1:50-1:100. For WB: Use at a dilution of 1:500-1:1,000. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | LKB1 (phospho S428), LKB1 (phospho Ser428), LKB1 (pS428), LKB1 (pSer428), LKB1 phospho S428, LKB1 phospho Ser428, Phospho LKB1 (S428), Phospho LKB1 (Ser428), Phospho LKB1 (pS428), Phospho LKB1 (pSer428) |
|---|---|
| Target | LKB1 (phospho Ser428) |
| Tissue Specificity | Detects endogenous levels of LKB1 only when phosphorylated at serine 428 |
Application
WB analysis of extracts from CV-1 cells treated with forsklin. Lane 1.) Using antibody and no peptide. Lane 2.) Using antibody preincubated with peptide.
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LKB1 (phospho-Ser428) antibody| Positive Control | Human breast carcinoma tissue, CV-1 cells treated with forsklin |
|---|---|
| Application Notes | For ELISA: Use at a dilution of 1:500-1:1,000. For IHC-P: Use at a dilution of 1:50-1:100. For WB: Use at a dilution of 1:500-1:1,000. Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS, pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol |
