Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using LMAN1 antibody at 1:500 dilution.Application
LMAN1 antibody [N1C1] detects LMAN1 protein at cytosol on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. LMAN1 antibody [N1C1] dilution: 1:500.| Product Name | LMAN1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LMAN1 (lectin, mannose-binding, 1)The protein encoded by this gene is a type I integral membrane protein localized in the intermediate region between the endoplasmic reticulum and the Golgi, presumably recycling between the two compartments. The protein is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined factor V-factor VIII deficiency, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LMAN1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chicken, Rhesus Monkey, BovineMouse (93%), Rat (93%), Chicken (81%), Rhesus Monkey (97%), Bovine (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ERGIC-53 antibody, ERGIC53 antibody, F5F8D antibody, FMFD1 antibody, MCFD1 antibody, MR60 antibody, gp58 antibody, LMAN1 antibody, endoplasmic reticulum-golgi intermediate compartment protein 53 antibody, protein ERGIC-53 antibody, ER-Golgi intermediate co |
|---|---|
| Molecular Weight(MW) | 58 kDa (note) (2) |
| Cellular Localization | Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein , Golgi apparatus membrane; Single-pass membrane protein , Endoplasmic reticulum membrane; Single-pass type I membrane protein |
Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using LMAN1 antibody at 1:500 dilution.
Application
LMAN1 antibody [N1C1] detects LMAN1 protein at cytosol on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. LMAN1 antibody [N1C1] dilution: 1:500.| Positive Control | HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
