Application
Sample A: 30μg of H1299 whole cell lysate B: 30μg of HeLa S3 whole cell lysate C: 30μg of Hep G2 whole cell lysate 7.5% SDS PAGE diluted at 1:1000Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using LOC113802 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using LOC113802 antibody at 1:500 dilution.| Product Name | LOC113802 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LOC113802 (HEN1 methyltransferase homolog 1 (Arabidopsis)) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LOC113802. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus Monkey, ChimpanzeeRhesus Monkey (99%), Chimpanzee (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | C1orf59 antibody, FLJ30525 antibody, HEN1 antibody, MGC111091 antibody, HENMT1 antibody, small RNA 2'-O-methyltransferase antibody, HEN1 methyltransferase homolog 1 (Arabidopsis) antibody |
|---|---|
| Molecular Weight(MW) | 45 kDa (note) (2) |
Application
Sample A: 30μg of H1299 whole cell lysate B: 30μg of HeLa S3 whole cell lysate C: 30μg of Hep G2 whole cell lysate 7.5% SDS PAGE diluted at 1:1000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using LOC113802 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using LOC113802 antibody at 1:500 dilution.| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
