Application
Immunofluorescence analysis of methanol-fixed A431, using LOK antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using LOK antibody at 1:250 dilution.Application
Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE diluted at 1:2000| Product Name | LOK antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LOK (serine/threonine kinase 10)This gene encodes a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. The protein can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. The kinase can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase kinase 1 pathway. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human LOK. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Chicken, BovineMouse (97%), Rat (97%), Zebrafish (82%), Xenopus laevis (84%), Chicken (90%), Bovine (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | LOK antibody, PRO2729 antibody, STK10 antibody, lymphocyte-oriented kinase antibody, serine/threonine-protein kinase 10 antibody, serine/threonine kinase 10 antibody |
|---|---|
| Molecular Weight(MW) | 112 kDa (note) |
Application
Immunofluorescence analysis of methanol-fixed A431, using LOK antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using LOK antibody at 1:250 dilution.
Application
Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE diluted at 1:2000| Positive Control | A431 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
