Application
Human Kidney Cortex (formalin-fixed, paraffin-embedded) stained with LRP2 antibody at 2.5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.| Product Name | LRP2 antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to LRP2The protein encoded by this gene, low density lipoprotein-related protein 2 (LRP2) or megalin, is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney. This glycoprotein has a large amino-terminal extracellular domain, a single transmembrane domain, and a short carboxy-terminal cytoplasmic tail. The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase. The LRP2 protein is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling; extracellular ligands include parathyroid horomones and the morphogen sonic hedgehog while cytosolic ligands include MAP kinase scaffold proteins and JNK interacting proteins. Recycling of this membrane receptor is regulated by phosphorylation of its cytoplasmic domain. Mutations in this gene cause Donnai-Barrow syndrome (DBS) and facio-oculoacoustico-renal syndrome (FOAR). |
| Immunogen | The immunogen corresponding a region within amino acids of 4641 and 4655 of human LRP2. |
| Clonality | Polyclonal |
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| Host Species | Rabbit |
| Tested Applications | ELISAIHC-P |
| Recommended Starting Dilutions: For ELISA: Use at a dilution of 1:1000. For IHC-P: Use at a concentration of 2.5 µg/ml. Optimal dilutions should be determined experimentally by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | DBS, GP330 |
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Application
Human Kidney Cortex (formalin-fixed, paraffin-embedded) stained with LRP2 antibody at 2.5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.| Application Notes | Recommended Starting Dilutions: For ELISA: Use at a dilution of 1:1000. For IHC-P: Use at a concentration of 2.5 µg/ml. Optimal dilutions should be determined experimentally by the end user. |
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| Form | Liquid |
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| Storage Instructions | Long term: -20°C; Short term: +4°C |
| Storage Buffer | PBS, 0.1% sodium azide. |
