Application
Western blot analysis of LRP-5/6 with Jurkat cell lysate (Lane 1: 100 µg; Lane 2: 50 µg).| Product Name | LRP-5/6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to LRP-5/6LDL-related proteins LRP-5 and LRP-6. are members of the LDL receptor superfamily, which were found to be involved in the canonical Wnt signaling pathway. Mammalian LRP-6 was shown to bind to Wnt-1 and enhance Induced-induced developmental processes in Xenopus embryos. Mice lacking LRP-6 exhibited developmental defects that are similar to those caused by deficiencies in various Want proteins. Recent work also shows that Want proteins induce the binding of LRP-5 to Axin, leading Axin degradation and beta-catenin stabilization. |
| Immunogen | immunogens is against the full length protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Jurkat cell lysate can be used as a positive control. The antibody recognizes the ~170 kDa full length LRP-5/6 of human and mouse origins. A cleavage fragment (~120 kDa) can also be detected. Reactivity to other species has not been tested. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | LRP-5, LRP6, LRP 6, LRP5, LRP -6, LRP 5, LRP 6 |
|---|---|
| Target | LRP-5/6 |
| Tissue Specificity | This antibody is specific for LRP-5/6. |
| Cellular Localization | Chaperoned to the plasma membrane by MESD. |
Application
Western blot analysis of LRP-5/6 with Jurkat cell lysate (Lane 1: 100 µg; Lane 2: 50 µg).| Application Notes | Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Jurkat cell lysate can be used as a positive control. The antibody recognizes the ~170 kDa full length LRP-5/6 of human and mouse origins. A cleavage fragment (~120 kDa) can also be detected. Reactivity to other species has not been tested. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal |
