Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-LRP6 (pS1490) RabMAb (cat. #) 1:1000 dilution. Cells were either (A) untreated (B) treated with Calyculin| Product Name | LRP6 (phospho Ser1490) antibody [EP2360Y] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EP2360Y] to LRP6 (phospho Ser1490)LRP6 (low-density lipoprotein receptor-related protein 6) is a single span transmembrane Wnt receptor that interacts with Axin to promote the Wnt-dependent accumulation of beta-catenin. Cells treated with Wnt quickly induce plasma membrane-associated LRP6 aggregates. The aggregates are phosphorylated, and then promote Axin recruitment and beta-catenin stabilization. LRP6 is internalized with caveolin and components of this endocytic pathway are required not only for Wnt-3a-induced internalization of LRP6 but also for accumulation of beta-catenin. Wnt-3a triggers the interaction of LRP6 with caveolin and promotes recruitment of Axin to LRP6 phosphorylated at Serine 1490 by glycogen synthase kinase-3beta . |
| Immunogen | A phospho specific peptide corresponding to residues surrounding serine 1490 of human LRP6 was used as an immunogen. |
| Modification | p-Ser1490 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EP2360Y |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| For WB: Use at a dilution of 1:500 - 1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Synonyms | LRP6, FLJ90421, FLJ90062, ADCAD2, LRP6 (phospho S1490), LRP6 (phospho Ser1490), LRP6 (pS1490), LRP6 (pSer1490), LRP6 phospho S1490, LRP6 phospho Ser1490, Phospho LRP6 (S1490), Phospho LRP6 (Ser1490), Phospho LRP6 (pS1490), Phospho LRP6 (pSer1490) |
|---|---|
| Target | LRP6 Phospho (pS1490) |
| Molecular Weight(MW) | 210 (note) |
| Tissue Specificity | This antibody detects LRP6 phosphorylated on serine 1490. |
Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-LRP6 (pS1490) RabMAb (cat. #) 1:1000 dilution. Cells were either (A) untreated (B) treated with Calyculin| Application Notes | For WB: Use at a dilution of 1:500 - 1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
