Application
RAP antibody [N1C3] detects RAP protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RAP protein stained by RAP antibody [N1C3] diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
RAP antibody [N1C3] detects RAP protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. RAP antibody [N1C3] diluted at 1:500.Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAP antibody [N1C3] diluted at 1:1000.Application
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAP antibody [N1C3] diluted at 1:1000.Application
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAP antibody [N1C3] diluted at 1:1000.| Product Name | LRPAP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to RAP (low density lipoprotein receptor-related protein associated protein 1)Interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LRPAP1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | ChimpanzeeChimpanzee (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | A2MRAP antibody, A2RAP antibody, HBP44 antibody, MGC138272 antibody, MRAP antibody, RAP antibody, LRPAP1 antibody, alpha-2-macroglobulin receptor-associated protein 1 antibody, low density lipoprotein-related protein-associated protein 1 (alpha-2-macroglob |
|---|---|
| Molecular Weight(MW) | 41 kDa (note) |
| Cellular Localization | Endoplasmic reticulum , Cytoplasm , Cell surface |
Application
RAP antibody [N1C3] detects RAP protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RAP protein stained by RAP antibody [N1C3] diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
RAP antibody [N1C3] detects RAP protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. RAP antibody [N1C3] diluted at 1:500.
Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAP antibody [N1C3] diluted at 1:1000.
Application
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAP antibody [N1C3] diluted at 1:1000.
Application
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAP antibody [N1C3] diluted at 1:1000.| Positive Control | A549 , HeLa , HepG2 , HCT116 , U87-MG , SK-N-SH , IMR32 , SK-N-AS |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
