Application
Immunocytochemistry/Immunofluorescence: LRRK2 Antibody [ - Mouse CAD cells transfected with Human wild-type LRRK-2 (1:2,000).Application
Immunohistochemistry: LRRK2 Antibody [ - Brain, Putamen, Neurons and Glia 60x.Application
Immunoprecipitation: LRRK2 Antibody [ - Immunoprecipitation of LRRK2 followed by an autophosphorylation assay using Lane 1 Rabbit IgG, Lanes 2 and 3Application
Western Blot: LRRK2 Antibody [ - Detection of LRRK2 in 50 ug of crude bovine brain membrane.Application
Western Blot: LRRK2 Antibody [ - Detection of LRRK2 in HeLa whole cell lysate (RIPA) using 1:5000 dilution, 1 minute ECL detection.| Product Name | LRRK2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to LRRK2 [Leucine-rich repeat kinase 2]LRRK2 is a 286 kDa protein belonging to the ROCO family. It is a multifunction protein involved in Parkinson's disease. |
| Immunogen | A C-terminal synthetic peptide made to the human LRRK2 protein sequence (between residues 2500-2527). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| The theoretical molecular weight of LRRK2 is ~286 kDa. We have also seen other bands with some lysates, but these bands have been blocked by the control peptide, suggesting that these other bands are degredation products. Optimal working dilutions should be determined by the investigator. Western Blot: 1:1,000~1:10,000 (varies depending upon sample)Immunofluorescence: 1:2,000 (Mouse CAD cells transfected with human wild-type LRRK-2)Not tested in any other application. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ROCO2, 120892, Q5S007, PARK8, LRRK2, 607060 |
|---|---|
| Target | LRRK2 |
| Tissue Specificity | reacts with LRRK2. |
Application
Immunocytochemistry/Immunofluorescence: LRRK2 Antibody [ - Mouse CAD cells transfected with Human wild-type LRRK-2 (1:2,000).
Application
Immunohistochemistry: LRRK2 Antibody [ - Brain, Putamen, Neurons and Glia 60x.
Application
Immunoprecipitation: LRRK2 Antibody [ - Immunoprecipitation of LRRK2 followed by an autophosphorylation assay using Lane 1 Rabbit IgG, Lanes 2 and 3
Application
Western Blot: LRRK2 Antibody [ - Detection of LRRK2 in 50 ug of crude bovine brain membrane.
Application
Western Blot: LRRK2 Antibody [ - Detection of LRRK2 in HeLa whole cell lysate (RIPA) using 1:5000 dilution, 1 minute ECL detection.| Application Notes | The theoretical molecular weight of LRRK2 is ~286 kDa. We have also seen other bands with some lysates, but these bands have been blocked by the control peptide, suggesting that these other bands are degredation products. Optimal working dilutions should be determined by the investigator. Western Blot: 1:1,000~1:10,000 (varies depending upon sample)Immunofluorescence: 1:2,000 (Mouse CAD cells transfected with human wild-type LRRK-2)Not tested in any other application. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4 degrees Celcius. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | This antibody is supplied as 0.1 ml of purified antibody [1 mg/ml] in Tris-citrate/phosphate [pH 7-8] containing 0.1% sodium azide. |
