Application
Immunofluorescence analysis of methanol-fixed MCF-7, using LRRK2 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using LRRK2 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: IMR32 5% SDS PAGE diluted at 1:1000| Product Name | LRRK2 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LRRK2 (leucine-rich repeat kinase 2)This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LRRK2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Pig, BovinePig (92%), Bovine (87%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AURA17 antibody, DARDARIN antibody, PARK8 antibody, RIPK7 antibody, ROCO2 antibody, LRRK2 antibody, leucine-rich repeat serine/threonine-protein kinase 2 antibody, augmented in rheumatoid arthritis 17 antibody, leucine-rich repeat kinase 2 antibody |
|---|---|
| Molecular Weight(MW) | 286 kDa (note) |
| Cellular Localization | Cytoplasm , Membrane; Peripheral membrane protein |
Application
Immunofluorescence analysis of methanol-fixed MCF-7, using LRRK2 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using LRRK2 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: IMR32 5% SDS PAGE diluted at 1:1000| Positive Control | NT2D1 , IMR32 , U87-MG , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
