Application
Flow cytometric analysis of HepG2 cells using LTA Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with LTA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of LTA Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). LTA (arrow) was detected using the purified Pab.| Product Name | LTA antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to LTA (Internal)The encoded protein, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4 and psoriatic arthritis. |
| Immunogen | KLH conjugated synthetic peptide between 52~81 amino acids from the Center region of human LTA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000 | |
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | TNF-beta, LTalpha, Lymphotoxinalpha, Lymphotoxin-alpha, TNFB, Tumor necrosis factor ligand superfamily member 1, LT-alpha, TNFbeta, Tumor necrosis factor ligand superfamily member1, LTA, TNFSF1, TNF beta, LT alpha, Lymphotoxin alpha |
|---|---|
| Target | LTA |
| Molecular Weight(MW) | 22.297 kDa (note) |
| Cellular Localization | The homotrimer is secreted. The heterotrimer is membrane-associated. |
Application
Flow cytometric analysis of HepG2 cells using LTA Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with LTA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of LTA Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). LTA (arrow) was detected using the purified Pab.| Application Notes | ELISA: Use at 1:1,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
