Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using LYK5 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: 293T B: HepG2 12% SDS PAGE diluted at 1:1000Application
LYK5 antibody detects LYK5 protein at cytoplasm by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LYK5 protein stained by LYK5 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | LYK5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LYK5 (STE20-related kinase adaptor alpha)The protein encoded by this gene contains a STE20-like kinase domain, but lacks several residues that are critical for catalytic activity, so it is termed a 'pseudokinase'. The protein forms a heterotrimeric complex with serine/threonine kinase 11 (STK11, also known as LKB1) and the scaffolding protein calcium binding protein 39 (CAB39, also known as MO25). The protein activates STK11 leading to the phosphorylation of both proteins and excluding STK11 from the nucleus. The protein is necessary for STK11-induced G1 cell cycle arrest. A mutation in this gene has been shown to result in polyhydramnios, megalencephaly, and symptomatic epilepsy (PMSE) syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their full-length nature is not known. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LYK5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Chicken, Sheep, Chimpanzee, BovineRat (95%), Chicken (92%), Sheep (96%), Chimpanzee (100%), Bovine (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ90524 antibody, LYK5 antibody, NY-BR-96 antibody, PMSE antibody, STRAD antibody, Stlk antibody, STRADA antibody, serologically defined breast cancer antigen NY-BR-96 antibody, STE20-related adapter protein antibody, STE20-like pseudokinase antibody, STR |
|---|---|
| Molecular Weight(MW) | 48 kDa (note) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using LYK5 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: 293T B: HepG2 12% SDS PAGE diluted at 1:1000
Application
LYK5 antibody detects LYK5 protein at cytoplasm by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LYK5 protein stained by LYK5 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
