Application
Immunofluorescence analysis of methanol-fixed A431, using LYPLA3 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using LYPLA3 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE diluted at 1:2000| Product Name | LYPLA3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LYPLA3 (phospholipase A2, group XV)Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene hydrolyzes lysophosphatidylcholine to glycerophosphorylcholine and a free fatty acid. This enzyme is present in the plasma and thought to be associated with high-density lipoprotein. A later paper contradicts the function of this gene. It demonstrates that this gene encodes a lysosomal enzyme instead of a lysophospholipase and has both calcium-independent phospholipase A2 and transacylase activities. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LYPLA3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Rhesus Monkey, BovineMouse (92%), Rat (91%), Dog (96%), Rhesus Monkey (100%), Bovine (85%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ACS antibody, DKFZp564A0122 antibody, GXVPLA2 antibody, LLPL antibody, LPLA2 antibody, LYPLA3 antibody, PLA2G15 antibody, lysophospholipase 3 (lysosomal phospholipase A2) antibody, lysosomal phospholipase A2 antibody, LCAT-like lysophospholipase antibody, |
|---|---|
| Molecular Weight(MW) | 47 kDa (note) (2) |
| Cellular Localization | Lysosome (By similarity) , Secreted |
Application
Immunofluorescence analysis of methanol-fixed A431, using LYPLA3 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using LYPLA3 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE diluted at 1:2000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
