Application
LYRIC antibody [N2C3] detects LYRIC protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. LYRIC antibody [N2C3] dilution: 1:500.Application
LYRIC antibody [N2C3] detects LYRIC protein at cytosol on human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. LYRIC antibody [N2C3] dilution: 1:500.Application
LYRIC antibody detects LYRIC protein by Western blot analysis. Rat tissue extracts (50 μg) was separated by 7.5 % SDS-PAGE, and the membrane was blotted with LYRIC antibody diluted by 1:1000.Application
LYRIC antibody [N2C3] detects LYRIC protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: LYRIC protein stained by LYRIC antibody [N2C3] diluted at 1:500. Blue: Hoechst 33342 staining.Application
LYRIC antibody [N2C3] detects LYRIC protein at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. LYRIC antibody [N2C3] dilution: 1:500.| Product Name | LYRIC antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to LYRIC (metadherin)Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LYRIC. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Rhesus Monkey, BovinePig (93%), Rhesus Monkey (98%), Bovine (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 3D3 antibody, AEG-1 antibody, AEG1 antibody, LYRIC antibody, LYRIC/3D3 antibody, MTDH antibody, protein LYRIC antibody, astrocyte elevated gene-1 protein antibody, metastasis adhesion protein antibody, 3D3/LYRIC antibody, lysine-rich CEACAM1 co-isolated pr |
|---|---|
| Target | LYRIC |
| Molecular Weight(MW) | 64 kDa (note) |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass membrane protein , Nucleus membrane; Single-pass membrane protein (By similarity) , Cell junction , tight junction (By similarity) , Nucleus , nucleolus (By similarity) , Cytoplasm , perinuclear region |
Application
LYRIC antibody [N2C3] detects LYRIC protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. LYRIC antibody [N2C3] dilution: 1:500.
Application
LYRIC antibody [N2C3] detects LYRIC protein at cytosol on human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. LYRIC antibody [N2C3] dilution: 1:500.
Application
LYRIC antibody detects LYRIC protein by Western blot analysis. Rat tissue extracts (50 μg) was separated by 7.5 % SDS-PAGE, and the membrane was blotted with LYRIC antibody diluted by 1:1000.
Application
LYRIC antibody [N2C3] detects LYRIC protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: LYRIC protein stained by LYRIC antibody [N2C3] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
LYRIC antibody [N2C3] detects LYRIC protein at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. LYRIC antibody [N2C3] dilution: 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
