Application
LYRIC antibody detects MTDH protein at endoplasmic reticulum and cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MTDH protein stained by LYRIC antibody diluted at 1:2500. Blue: Hoechst 33342 staining.Application
LYRIC antibody detects LYRIC protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. LYRIC antibody diluted at 1:2400.Application
LYRIC antibody detects LYRIC protein at cytoplasm in rat intestine by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. LYRIC antibody diluted at 1:2400.Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LYRIC antibody diluted at 1:1000.| Product Name | LYRIC antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to LYRIC (metadherin)Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LYRIC. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Pig, Rhesus Monkey, BovineMouse (93%), Pig (93%), Rhesus Monkey (98%), Bovine (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AEG1 , metadherin , 3D3 , LYRIC/3D3 , MTDH |
|---|---|
| Target | LYRIC |
| Molecular Weight(MW) | 64 kDa (note) |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass membrane protein , Nucleus membrane; Single-pass membrane protein (By similarity) , Cell junction , tight junction (By similarity) , Nucleus , nucleolus (By similarity) , Cytoplasm , perinuclear region |
Application
LYRIC antibody detects MTDH protein at endoplasmic reticulum and cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MTDH protein stained by LYRIC antibody diluted at 1:2500. Blue: Hoechst 33342 staining.
Application
LYRIC antibody detects LYRIC protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. LYRIC antibody diluted at 1:2400.
Application
LYRIC antibody detects LYRIC protein at cytoplasm in rat intestine by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. LYRIC antibody diluted at 1:2400.
Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LYRIC antibody diluted at 1:1000.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
