Application
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using Lysyl-tRNA synthetase antibody at 1:100 dilution.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Lysyl-tRNA synthetase antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Lysyl-tRNA synthetase antibody detects Lysyl-tRNA synthetase protein at cytosol on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Lysyl-tRNA synthetase antibody dilution: 1:500.| Product Name | Lysyl-tRNA synthetase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Lysyl-tRNA synthetase (lysyl-tRNA synthetase)Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Lysyl-tRNA synthetase. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Chicken, Rhesus Monkey, BovineRat (93%), Zebrafish (84%), Xenopus laevis (87%), Chicken (85%), Rhesus Monkey (96%), Bovine (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CMTRIB antibody, KARS2 antibody, KIAA0070 antibody, KRS antibody, KARS antibody, lysRS antibody, lysyl-tRNA synthetase antibody, lysine tRNA ligase antibody |
|---|---|
| Molecular Weight(MW) | 68 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using Lysyl-tRNA synthetase antibody at 1:100 dilution.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Lysyl-tRNA synthetase antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Lysyl-tRNA synthetase antibody detects Lysyl-tRNA synthetase protein at cytosol on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Lysyl-tRNA synthetase antibody dilution: 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
