Rabbit polyclonal to M13 Bacteriophage Coat ProteinsMay act as a capture antibody when coated directly on microtiter plates, or as a primary detection antibody for specifically captured M13 or fd phage. May be useful in rapidly sorting large phage display libraries (antibody, peptide etc.) with the expressed proteins fused to either the gene III or gene VIII protein of the filamentous phage. May be used as a reagent in ""phage ELISA"" offering sensitive and specific activity for detection of recombinant phages.
Immunogen
fd bacteriophage
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ELISAICC/IF
IF: Use at an assay dependant dilution. I-ELISA: at least 1/1000 for 5 x10^7 phage/mlat least 1/8000 for 5 x 10^10 phage/ml Optimal dilutions/concentrations should be determined by the end user.
Concentration
1mg/ml
Purification
Unpurified
Target Information
Target
M13
Tissue Specificity
Binds specifically to phage coat proteins of M13 or fd phage.
Application Notes
IF: Use at an assay dependant dilution. I-ELISA: at least 1/1000 for 5 x10^7 phage/mlat least 1/8000 for 5 x 10^10 phage/ml Optimal dilutions/concentrations should be determined by the end user.