Application
Western Blot of acid extracts from K562 cells, using anti-Histone macroH2A1 Rabbit mAb RM248 at 1 ug/mL.Application
Immunocytochemistry of HeLa cells, using anti-Histone macroH2A1 Rabbit mAb RM248 (red). Actin filaments have been labeled with fluorescein phalloidin (green).| Product Name | Macro H2A.1 antibody [RM248] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM248] to Macro H2A.1Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Immunogen | A peptide corresponding to the C-terminus of human Histone macroH2A1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM248 |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWBMultiplexing |
| Western Blot: 0.5 ug/mL – 2 ug/mL, ICC: 1 μg/mL - 2 μg/mL, ELISA: 0.2 ug/mL - 1 ug/mL, Multiplex: 0.2 ug/mL – 1 ug/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | mH2A1 , H2AF12M , H2AFJ , MACROH2A1.1 , H2A/y , H2A.y , H2AFY , H2A histone family member Y , macroH2A1.2 |
|---|---|
| Target | Macro H2A.1 |
| Tissue Specificity | This antibody reacts to the Core histone macro-H2A.1 (Histone macroH2A1) protein, independent of post-translational modifications. No cross reactivity with other histone proteins. |
Application
Western Blot of acid extracts from K562 cells, using anti-Histone macroH2A1 Rabbit mAb RM248 at 1 ug/mL.
Application
Immunocytochemistry of HeLa cells, using anti-Histone macroH2A1 Rabbit mAb RM248 (red). Actin filaments have been labeled with fluorescein phalloidin (green).| Application Notes | Western Blot: 0.5 ug/mL – 2 ug/mL, ICC: 1 μg/mL - 2 μg/mL, ELISA: 0.2 ug/mL - 1 ug/mL, Multiplex: 0.2 ug/mL – 1 ug/mL. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20.0°C |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
