Application
Immunoprecipitation of MAD1 protein from HeLa whole cell extracts using 5 μg of MAD1 antibody . Western blot analysis was performed using MAD1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
MAD1 antibody detects MAD1L1 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract E. 30 µg A375 whole cell lysate/extract 7.5% SDS-PAGE MAD1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using MAD1 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using MAD1 antibody at 1:500 dilution.| Product Name | MAD1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MAD1 (MAD1 mitotic arrest deficient-like 1 (yeast))MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MAD1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (80%), Rat (81%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MAD1 antibody, PIG9 antibody, TP53I9 antibody, TXBP181 antibody, MAD1L1 antibody, mitotic arrest deficient 1-like protein 1 antibody, mitotic spindle assembly checkpoint protein MAD1 antibody, mitotic checkpoint MAD1 protein homolog antibody, tax-binding p |
|---|---|
| Molecular Weight(MW) | 83 kDa (note) (5) |
| Cellular Localization | Nucleus , Kinetochore |
Application
Immunoprecipitation of MAD1 protein from HeLa whole cell extracts using 5 μg of MAD1 antibody . Western blot analysis was performed using MAD1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
MAD1 antibody detects MAD1L1 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract E. 30 µg A375 whole cell lysate/extract 7.5% SDS-PAGE MAD1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using MAD1 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using MAD1 antibody at 1:500 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
