| Product Name | MAD2L1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MAD2L1MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq] |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| Western blotting (1:500 – 1:2000) and Immunoprecipitation. HeLa cell lysate can be used as a positive control. However, the optimal concentrations should be determined individually. The antibody recognizes the MAD2 of human origin. Reactivity to other species has not been tested. Reactivity with mouse samples is likely due to high sequence homology. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MAD2, MAD2L1, MAD2-like 1, Mitotic Arrest Deficient2, MAD2like 1, Mitotic Arrest Deficient 2, MAD2like1, HsMAD2, MAD2 like 1 |
|---|---|
| Target | MAD2 |
| Tissue Specificity | This antibody is specific for MAD2. |
| Cellular Localization | Recruited by MAD1L1 to unattached kinetochores (Probable). Recruited to the nuclear pore complex by TPR during interphase. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of |
| Application Notes | Western blotting (1:500 – 1:2000) and Immunoprecipitation. HeLa cell lysate can be used as a positive control. However, the optimal concentrations should be determined individually. The antibody recognizes the MAD2 of human origin. Reactivity to other species has not been tested. Reactivity with mouse samples is likely due to high sequence homology. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 1% BSA and 0.02% thimerosal |
