Application
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Madd antibody diluted at 1:1000.| Product Name | Madd antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Madd (MAP-kinase activating death domain)Plays a significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis). |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of mouse madd. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 9630059K23Rik antibody , IG20 antibody , MAP kinase-activating death domain protein antibody , MAP-kinase activating death domain antibody , Madd antibody , Madd antibody , Rab3 GDP/GTP exchange protein antibody , Rab3 GEP antibody , insulinoma-glu |
|---|---|
| Target | Madd |
| Molecular Weight(MW) | 175 kDa (note) (3) |
| Cellular Localization | Membrane; Multi-pass membrane protein |
Application
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Madd antibody diluted at 1:1000.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
