Application
Western blot analysis of MADD in whole cell lysates from the indicated cell lines with Anti-MADD at 1:250 dilution.Application
Western blot analysis of whole cell lysates from the indicated cell lines probed with Rabbit anti Human MADD| Product Name | MADD antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MADDMAP kinase-activating death domain protein (MADD) was initially identified as the type 1 tumor necrosis factor receptor (TNFR1) associated protein though their death domains (1). Overexpression of MADD activates MAP kinases ERK and JNK and induces the phosphorylation of cytosolic phospholipase A2 (1). MADD shares 98% identity with DENN (for differentially expressed in neoplastic versus normal cells), which was recently identified as a substrate for c-jun N-terminal kinase 3 (JNK3) (2, 3). MADD has greater than 94% overall identity to a GDP/GTP exchange protein Rab3-GEP (4, 5). MADD is 87% identical toKIAA0358, a brain protein of unknown function (4). Identification of MADD as a component of the TNFR1signaling complex and the similarity between MADD andRab3-GEP provides a connection between TNFR1activation and downstream MAP kinase activity through a guanine-nucleotide exchange protein. |
| Immunogen | Synthetic peptide - amino acids 1570 to 1588 of human MADD. This sequence is identical to that of DENN and differs by one amino acid with rat GDP/GTP exchange protein RAb3-GEP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB: Use at a dilution of 1:250-1:500. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IG20, FLJ35600, DENN, KIAA0358, RAB3GEP, MADD, FLJ36300 |
|---|---|
| Target | MADD |
Application
Western blot analysis of MADD in whole cell lysates from the indicated cell lines with Anti-MADD at 1:250 dilution.
Application
Western blot analysis of whole cell lysates from the indicated cell lines probed with Rabbit anti Human MADD| Application Notes | For WB: Use at a dilution of 1:250-1:500. Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 0.02% sodium azide |
