Application
Western blot analysis of Smad7 expression in Jurkat cell lysate (Lane 1& 2) and 3T3 cell lysate (Lane 3).| Product Name | MADH7 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MADH7Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad), have been implicated as downstream effectors of TGFbeta/BMP signaling. Smad1, Smad5, and Smad8 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF-beta and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFbeta signaling by interfering with TGFbeta-mediated phosphorylation of other Smad family members. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Other applications have not been determined. The antibody detects ~46 kDa Smad-7 from samples of human, mouse, and rat origins. Reactivity to other species has not been determined. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Smad7, hSMAD7, SMAD family member7, SMAD7, MADH8, MADH7, Smad7, hSMAD7, SMAD family member 7 |
|---|---|
| Target | Smad7 |
| Tissue Specificity | This antibody is specific for Smad7. |
| Cellular Localization | Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm. |
Application
Western blot analysis of Smad7 expression in Jurkat cell lysate (Lane 1& 2) and 3T3 cell lysate (Lane 3).| Application Notes | Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Other applications have not been determined. The antibody detects ~46 kDa Smad-7 from samples of human, mouse, and rat origins. Reactivity to other species has not been determined. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20ºC. The antibody solution should be gently mixed before use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal |
