Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using MAEA antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:5000| Product Name | MAEA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MAEA (macrophage erythroblast attacher)This gene product mediates the attachment of erythroblasts to macrophages. This attachment promotes terminal maturation and enucleation of erythroblasts, presumably by suppressing apoptosis. This protein is an integral membrane protein with the N-terminus on the extracellular side and the C-terminus on the cytoplasmic side of the cell. Two immunologically related isoforms of erythroblast macrophage protein with apparent molecular weights of 33 kD and 36 kD were detected in macrophage membranes; this gene encodes the larger isoform. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MAEA. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Chicken, Rhesus Monkey, BovineMouse (98%), Rat (98%), Zebrafish (93%), Xenopus laevis (95%), Chicken (95%), Rhesus Monkey (100%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EMLP antibody, EMP antibody, HLC-10 antibody, PIG5 antibody, MAEA antibody, cell proliferation-inducing gene 5 protein antibody, macrophage erythroblast attacher antibody, proliferation-inducing gene 5 antibody, erythroblast macrophage protein antibody, lu |
|---|---|
| Molecular Weight(MW) | 45 kDa (note) (6) |
| Cellular Localization | Nucleus matrix , Cell membrane , Cytoplasm , cytoskeleton |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using MAEA antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:5000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
