Application
MAF1 antibody detects MAF1 protein at nucleus on human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. MAF1 antibody dilution: 1:500.Application
MAF1 antibody detects MAF1 protein at nucleus and cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. MAF1 antibody dilution: 1:500.Application
Sample(30 µg whole cell lysate) A:A431 B:MOLT4 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
MAF1 antibody detects MAF1 protein by western blot analysis. A. 30 µg GL261 whole cell lysate/extract B. 30 µg BCL-1 whole cell lysate/extract C. 30 µg Raw264.7 whole cell lysate/extract 12% SDS-PAGE MAF1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
MAF1 antibody detects MAF1 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 12% SDS-PAGE MAF1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MAF1 antibody at 1:200 dilution.| Product Name | MAF1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MAF1 (MAF1 homolog (S. cerevisiae))This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MAF1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Rhesus Monkey, BovineXenopus laevis (86%), Rhesus Monkey (98%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC20332 antibody, MGC31779 antibody, MGC39758 antibody, MAF1 antibody, repressor of RNA polymerase III transcription MAF1 homolog antibody, homolog of yeast MAF1 antibody, MAF1 homolog (S. cerevisiae) antibody |
|---|---|
| Molecular Weight(MW) | 29 kDa (note) |
| Cellular Localization | Nucleus |
Application
MAF1 antibody detects MAF1 protein at nucleus on human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. MAF1 antibody dilution: 1:500.
Application
MAF1 antibody detects MAF1 protein at nucleus and cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. MAF1 antibody dilution: 1:500.
Application
Sample(30 µg whole cell lysate) A:A431 B:MOLT4 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
MAF1 antibody detects MAF1 protein by western blot analysis. A. 30 µg GL261 whole cell lysate/extract B. 30 µg BCL-1 whole cell lysate/extract C. 30 µg Raw264.7 whole cell lysate/extract 12% SDS-PAGE MAF1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
MAF1 antibody detects MAF1 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 12% SDS-PAGE MAF1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MAF1 antibody at 1:200 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
