Application
MAFF antibody detects MAFF protein at cytoplasm and nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. MAFF antibody diluted at 1:250.Application
MAFF antibody detects MAFF protein by Western blot analysis. A. 30 µg Huh7 whole cell lysate/extract B. 30 µg HepG2 whole cell lysate/extract 12 % SDS-PAGE MAFF antibody dilution: 1:2000Application
MAFF antibody detects MAFF protein by western blot analysis. Non-transfected (-) and MAFF -transfected (+, ) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MAFF antibody diluted by 1:20000.| Product Name | MAFF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MAFF (v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian))The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. The encoded protein can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. This gene may also be involved in the cellular stress response. Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MAFF. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chicken, BovineMouse (97%), Rat (97%), Chicken (85%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian), U-MAF, hMafF |
|---|---|
| Target | MAFF |
| Cellular Localization | Nucleus |
Application
MAFF antibody detects MAFF protein at cytoplasm and nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. MAFF antibody diluted at 1:250.
Application
MAFF antibody detects MAFF protein by Western blot analysis. A. 30 µg Huh7 whole cell lysate/extract B. 30 µg HepG2 whole cell lysate/extract 12 % SDS-PAGE MAFF antibody dilution: 1:2000
Application
MAFF antibody detects MAFF protein by western blot analysis. Non-transfected (-) and MAFF -transfected (+, ) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MAFF antibody diluted by 1:20000.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
