Application
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with MAFG antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded PC9 xenograft, using MAFG antibody at 1:500 dilution.Application
MAFG antibody detects MAFG protein by western blot analysis. A.50 µg mouse muscle lysate/extract 12% SDS-PAGE MAFG antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
MAFG antibody detects MAFG protein by western blot analysis. A.50 µg rat muscle lysate/extract 12% SDS-PAGE MAFG antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
MAFG antibody detects MAFG protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAFG protein stained by MAFG antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.Application
Immunoprecipitation of MAFG protein from HeLa whole cell extracts using 5 μg of MAFG antibody . Western blot analysis was performed using MAFG antibody . EasyBlot anti-Mouse IgG was used as a secondary reagent.| Product Name | MAFG antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MAFG (v-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian))Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters (summarized by Blank et al., 1997 [PubMed 9166829]). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MAFG. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Chicken, Rhesus Monkey, BovineChicken (92%), Rhesus Monkey (100%), Bovine (98%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC13090 antibody, MGC20149 antibody, MAFG antibody, transcription factor MafG antibody, basic leucine zipper transcription factor MafG antibody, v-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian) antibody |
|---|---|
| Molecular Weight(MW) | 18 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with MAFG antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded PC9 xenograft, using MAFG antibody at 1:500 dilution.
Application
MAFG antibody detects MAFG protein by western blot analysis. A.50 µg mouse muscle lysate/extract 12% SDS-PAGE MAFG antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
MAFG antibody detects MAFG protein by western blot analysis. A.50 µg rat muscle lysate/extract 12% SDS-PAGE MAFG antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
MAFG antibody detects MAFG protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAFG protein stained by MAFG antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.
Application
Immunoprecipitation of MAFG protein from HeLa whole cell extracts using 5 μg of MAFG antibody . Western blot analysis was performed using MAFG antibody . EasyBlot anti-Mouse IgG was used as a secondary reagent.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
