Application
Cross-linked ChIP was performed with HepG2 chromatin extract treated with LPS (10 ng/ml for 1 h) and 5 μg of either normal rabbit IgG or anti-MAFK antibody. The precipitated DNA was detected by PCR with primer set targeting to IL-8 or GAPDH .Application
MAFK antibody immunoprecipitates MAFK protein in IP experiments. IP samples: Jurkat whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of MAFK protein by 4 μg MAFK antibody 15 % SDS-PAGE The immunoprecipitated MAFK protein was detected by MAFK antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
MAFK antibody detects MAFK protein by western blot analysis. A. 30 μg Jurkat whole cell lysate/extract 15 % SDS-PAGE MAFK antibody dilution: 1:500| Product Name | MAFK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MAFK (v-maf musculoaponeurotic fibrosarcoma oncogene homolog K (avian))The developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locus control regions (LCRs). LCRs are associated with powerful enhancer activity that is mediated by the transcription factor NFE2 (nuclear factor erythroid-2). NFE2 recognition sites are also present in the gene promoters of 2 heme biosynthetic enzymes, porphobilinogen deaminase (PBGD; MIM 609806) and ferrochelatase (FECH; MIM 612386). NFE2 DNA-binding activity consists of a heterodimer containing an 18-kD Maf protein (MafF, MafG (MIM 602020), or MafK) and p45 (MIM 601490). Both subunits are members of the activator protein-1 superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160). Maf homodimers suppress transcription at NFE2 sites.[supplied by OMIM] |
| Immunogen | Full length human MAFK Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Chicken, BovineMouse (98%), Rat (97%), Zebrafish (83%), Chicken (93%), Bovine (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | v-maf musculoaponeurotic fibrosarcoma oncogene homolog K (avian), P18, NFE2U |
|---|---|
| Target | MAFK |
| Molecular Weight(MW) | 18 kDa (note) |
| Cellular Localization | Nucleus |
Application
Cross-linked ChIP was performed with HepG2 chromatin extract treated with LPS (10 ng/ml for 1 h) and 5 μg of either normal rabbit IgG or anti-MAFK antibody. The precipitated DNA was detected by PCR with primer set targeting to IL-8 or GAPDH .
Application
MAFK antibody immunoprecipitates MAFK protein in IP experiments. IP samples: Jurkat whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of MAFK protein by 4 μg MAFK antibody 15 % SDS-PAGE The immunoprecipitated MAFK protein was detected by MAFK antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
MAFK antibody detects MAFK protein by western blot analysis. A. 30 μg Jurkat whole cell lysate/extract 15 % SDS-PAGE MAFK antibody dilution: 1:500| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
