Application
Immunocytochemistry of human melanoma cells (Formalin fixed/Paraffin-embedded) stained with| Product Name | MAGE 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MAGE 1The MAGE1 gene is a member of the melanoma antigen-encoding gene family. These genes encode for HLA-restricted tumor-associated rejection antigens recognized by cytotoxic T lymphocytes. Some of these target antigens may be potentially useful for cancer-specific immunotherapy. The expression of MAGE genes has been reported not only in melanoma but also in various other malignant tumors such as hepatocellular carcinoma and germ cell tumors. |
| Immunogen | Synthetic peptide: QEKYLEYRQVPDSDP, corresponding to amino acids 245-259 of Human MAGE 1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-FIPWB |
| ELISA: Use at a concentration of 0.1 - 1 μg/ml. IHC-F: 1/100 for 10 min. Note: prior staining, formalin-fixed tissues need to be boiled in 10 mM citrate buffer, pH 6.0 for 10 min, followed by cooling at RT for 20 min. IP: Use 3 - 5μg for extract from 10^7 cells. WB: Use at a concentration of 0.5 - 2 μg/ml. Predicted molecular weight: 34 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Melanoma antigen 1, MAGE 1 antigen, Melanoma antigen1, Melanoma associated antigen1, Antigen MZ2 E, MAGE 1, MAGE1, Melanoma associated antigen 1, MAGE1 antigen, CT1.1, MGC9326 |
|---|---|
| Target | MAGE 1 |
| Cellular Localization | Cell Membrane |
Application
Immunocytochemistry of human melanoma cells (Formalin fixed/Paraffin-embedded) stained with| Application Notes | ELISA: Use at a concentration of 0.1 - 1 μg/ml. IHC-F: 1/100 for 10 min. Note: prior staining, formalin-fixed tissues need to be boiled in 10 mM citrate buffer, pH 6.0 for 10 min, followed by cooling at RT for 20 min. IP: Use 3 - 5μg for extract from 10^7 cells. WB: Use at a concentration of 0.5 - 2 μg/ml. Predicted molecular weight: 34 kDa. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide. Constituents: PBS. pH 7.4 |
