Application
MAGEA4 antibody detects MAGEA4 protein at cytoplasm by immunofluorescent analysis. Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAGEA4 protein stained by MAGEA4 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE diluted at 1:2000Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using MAGEA4 antibody at 1:500 dilution.| Product Name | MAGEA4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MAGEA4 (melanoma antigen family A, 4)This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. At least four variants encoding the same protein have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MAGEA4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CT1.4 antibody, MAGE-41 antibody, MAGE-X2 antibody, MAGE4 antibody, MAGE4A antibody, MAGE4B antibody, MGC21336 antibody, MAGEA4 antibody, MAGE-X2 antigen antibody, MAGE-41 antigen antibody, MAGE-4 antigen antibody, melanoma-associated antigen 4 antibody, c |
|---|---|
| Molecular Weight(MW) | 35 kDa (note) (5) |
Application
MAGEA4 antibody detects MAGEA4 protein at cytoplasm by immunofluorescent analysis. Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAGEA4 protein stained by MAGEA4 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE diluted at 1:2000
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using MAGEA4 antibody at 1:500 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
