Application
MBP antibody Immunoprecipitation of MBP tag protein. E. coli lysates were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 0.5 ug of anti-MBP antibody . (A) Input, 0.2ug of E.coli lysates. The precipitated protein was detected by diluted at 1:2000. EasyBlot anti-Rabbit IgG Kit was used in Western blot.Application
Sample (0.5ul of whole cell lysate) A: E.coli non-induction B: E.coli induction with 1mM IPTG 10% SDS PAGE diluted at 1:50000| Product Name | Maltose binding protein antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MBP (Maltose-Binding Protein) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MBP, Maltose-Binding Protein |
|---|
Application
MBP antibody Immunoprecipitation of MBP tag protein. E. coli lysates were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 0.5 ug of anti-MBP antibody . (A) Input, 0.2ug of E.coli lysates. The precipitated protein was detected by diluted at 1:2000. EasyBlot anti-Rabbit IgG Kit was used in Western blot.
Application
Sample (0.5ul of whole cell lysate) A: E.coli non-induction B: E.coli induction with 1mM IPTG 10% SDS PAGE diluted at 1:50000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 1%BSA, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
