Application
WB analysis of 293T cells using MANEA antibody at 0.2-1μg/ml.Application
WB analysis of human fetal heart tissue using MANEA antibody at 1μg/ml.| Product Name | MANEA antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MANEAN-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005 [PubMed 15677381]).[supplied by OMIM, Sep 2008] |
| Immunogen | A synthetic peptide corresponding to an Internal region of Human MANEA |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Dog: 100%; Horse: 86%; Human: 100%; Mouse: 100%; Pig: 93%; Rabbit: 92%; Rat: 100%; Zebrafish: 77% |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ENDO , HENDO , MANEA |
|---|---|
| Target | MANEA |
| Molecular Weight(MW) | 54 KDa (note) |
Application
WB analysis of 293T cells using MANEA antibody at 0.2-1μg/ml.
Application
WB analysis of human fetal heart tissue using MANEA antibody at 1μg/ml.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
