Application
Western blot detection of Mek1 protein in RAW cell (1) and RBL transfected with NIK(2).| Product Name | MAP2K1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MAP2K1MAP2K1, MEK1 (Mitogen activated protein kinase kinase 1) catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. MEK1 activates ERK1 and ERK2 MAP kinases. Mitogen activated protein kinase kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK1 and 2 and MAPK enzymes by phosphorylating both residues within the threonine/glutamate/tyrosine (TEY) motif in the activation loop. MEK1 and 2 are also activated by dual phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEK. Threonine 292 of MEK1 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK1. |
| Immunogen | Recombinant protein - full length mouse MAP2K1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| For WB: Use at a dilution of 1:2,000. For IP: Use at a dilution of 1:2,000. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MAPKK1, MEK1, MKK1, PRKMK1 |
|---|---|
| Target | MAP2K1 |
| Tissue Specificity | MAP2K1 in RAW cell and RBL transfected with NIK |
Application
Western blot detection of Mek1 protein in RAW cell (1) and RBL transfected with NIK(2).| Application Notes | For WB: Use at a dilution of 1:2,000. For IP: Use at a dilution of 1:2,000. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline, pH 7.2, containing 0.1% sodium azide |
