Application
Western blot analysis of extracts from JK cells (Lane 2), using MAP3K1 (Phospho-Thr1400) Antibody. Lane 1 on the left is treated with antigen-specific peptide.Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP3K1 (Phospho-Thr1400) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | MAP3K1 (phospho Thr1402) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MAP3K1 (phospho Thr1402)The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades, including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins. This protein has E3 ligase activity conferred by a plant homeodomain (PHD) in its N-terminus and phospho-kinase activity conferred by a kinase domain in its C-terminus. [provided by RefSeq, Mar 2012] |
| Immunogen | Peptide sequence around phosphorylation site of threonine 1400(K-G-T(p)-G-A) derived from Human MAP3K1. |
| Modification | p-Thr1402 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:1000. IHC: 1:50 - 1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | SRXY6 , MEKK , MEKK1 , MAPKKK1 , MEKK 1 , mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase , MAP3K1 |
|---|---|
| Target | MAP3K1 (phospho Thr1402) |
| Tissue Specificity | The antibody detects endogenous levels of MAP3K1 only when phosphorylated at threonine 1400. |
Application
Western blot analysis of extracts from JK cells (Lane 2), using MAP3K1 (Phospho-Thr1400) Antibody. Lane 1 on the left is treated with antigen-specific peptide.
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP3K1 (Phospho-Thr1400) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | WB: 1:500 - 1:1000. IHC: 1:50 - 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C/1 year |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
