Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP3K8 (Phospho-Ser400) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | MAP3K8 (phospho Ser400) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MAP3K8 (phospho Ser400)This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011] |
| Immunogen | Peptide sequence around phosphorylation site of Serine 400(C-Q-S(p)-L-D) derived from HumanMAP3K8. |
| Modification | p-Ser400 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| IHC: 1:50 - 1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MEKK8 EST Tpl-2 MAP3K8 ESTF c-COT mitogen-activated protein kinase kinase kinase 8 AURA2 COT TPL2 |
|---|---|
| Target | MAP3K8 (phospho Ser400) |
| Tissue Specificity | The antibody detects endogenous levels of MAP3K8 only when phosphorylated at serine 400. |
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP3K8 (Phospho-Ser400) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | IHC: 1:50 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C/1 year |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
