Application
Sample (30 µg of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of methanol-fixed HeLa, using MAP3K8 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using MEKK8(COT) antibody at 1:100 dilution.Application
MAP3K8 antibody [N3C3] detects MAP3K8 protein at cytoplasm on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. MAP3K8 antibody [N3C3] diluted at 1:100.| Product Name | MAP3K8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MAP3K8 (mitogen-activated protein kinase kinase kinase 8)This gene was identified by its oncogenic transforming activity in cells. The encoded protein is a member of the serine/threonine protein kinase family. This kinase can activate both the MAP kinase and JNK kinase pathways. This kinase was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This kinase was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. Studies of a similar gene in rat suggested the direct involvement of this kinase in the proteolysis of NF-kappaB1,p105 (NFKB1). This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MAP3K8. The exact sequence is proprietary. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB:1:500~1:2000 ICC/IF:1:100~1:500 IHC-p:1:50~1:200 |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (95%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | COT antibody EST antibody ESTF antibody FLJ10486 antibody MEKK8 antibody TPL2 antibody Tpl-2 antibody c-COT antibody MAP3K8 antibody mitogen-activated protein kinase kinase kinase 8 antibody Ewing sarcoma transformant antibody tumor progression |
|---|---|
| Molecular Weight(MW) | 53 kDa (note) (3) |
Application
Sample (30 µg of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using MAP3K8 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using MEKK8(COT) antibody at 1:100 dilution.
Application
MAP3K8 antibody [N3C3] detects MAP3K8 protein at cytoplasm on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. MAP3K8 antibody [N3C3] diluted at 1:100.| Positive Control | A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | WB:1:500~1:2000 ICC/IF:1:100~1:500 IHC-p:1:50~1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
