Application
MAP4 antibody detects MAP4 protein at cytoskeleton by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: MAP4 protein stained by MAP4 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
MAP4 antibody detects MAP4 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 5 % SDS-PAGE MAP4 antibody dilution: 1:5000Application
MAP4 antibody detects MAP4 protein at cytoplasm on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. MAP4 antibody diluted at 1:250.| Product Name | MAP4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MAP4 (microtubule-associated protein 4)The protein encoded by this gene is a major non-neuronal microtubule-associated protein. This protein contains a domain similar to the microtubule-binding domains of neuronal microtubule-associated protein (MAP2) and microtubule-associated protein tau (MAPT/TAU). This protein promotes microtubule assembly, and has been shown to counteract destabilization of interphase microtubule catastrophe promotion. Cyclin B was found to interact with this protein, which targets cell division cycle 2 (CDC2) kinase to microtubules. The phosphorylation of this protein affects microtubule properties and cell cycle progression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Full length human MAP4 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Cat, Rhesus Monkey, BovineCat (84%), Rhesus Monkey (97%), Bovine (82%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | microtubule-associated protein 4 |
|---|---|
| Target | MAP4 |
| Molecular Weight(MW) | 121 kDa (note) |
Application
MAP4 antibody detects MAP4 protein at cytoskeleton by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: MAP4 protein stained by MAP4 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
MAP4 antibody detects MAP4 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 5 % SDS-PAGE MAP4 antibody dilution: 1:5000
Application
MAP4 antibody detects MAP4 protein at cytoplasm on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. MAP4 antibody diluted at 1:250.| Positive Control | 293T , A431 , HeLa , HepG2 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
