Application
MAP4K6 antibody [C2C3-2], C-term detects MAP4K6 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: MAP4K6 protein stained by MAP4K6 antibody [C2C3-2], C-term diluted at 1:500. Blue: Hoechst 33342 staining.Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000Application
MAP4K6 antibody [C2C3-2], C-term detects MINK1 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. MAP4K6 antibody [C2C3-2], C-term dilution: 1:500.| Product Name | MAP4K6 antibody , C-term |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MAP4K6 (misshapen-like kinase 1)This gene encodes a serine/threonine kinase belonging to the germinal center kinase (GCK) family. The protein is structurally similar to the kinases that are related to NIK and may belong to a distinct subfamily of NIK-related kinases within the GCK family. Studies of the mouse homolog indicate an up-regulation of expression in the course of postnatal mouse cerebral development and activation of the cJun N-terminal kinase (JNK) and the p38 pathways. Alternative splicing occurs at this locus and four transcript variants encoding distinct isoforms have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human MAP4K6. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, BovineMouse (97%), Rat (97%), Zebrafish (81%), Xenopus laevis (86%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | B55 antibody MAP4K6 antibody MGC21111 antibody MINK antibody YSK2 antibody ZC3 antibody hMINK antibody hMINKbeta antibody MINK1 antibody serine/threonine protein kinase antibody misshapen-like kinase 1 antibody mitogen-activated protein kinase k |
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| Molecular Weight(MW) | 150 kDa (note) (5) |
Application
MAP4K6 antibody [C2C3-2], C-term detects MAP4K6 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: MAP4K6 protein stained by MAP4K6 antibody [C2C3-2], C-term diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000
Application
MAP4K6 antibody [C2C3-2], C-term detects MINK1 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. MAP4K6 antibody [C2C3-2], C-term dilution: 1:500.| Positive Control | HepG2 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
