Application
WB analysis of lysates from (A, C) zebrafish embryo (24 hpf) and (B, D) zebrafish larva (6 dpf) using Mapk3 antibody in the (A) absence or (B) presence of blocking peptide.Application
IP analysis was perform with zebrafish embryonic (24-48 hpf) cytosolic lysate and either (B) Mapk3 antibody or (C) immunizing peptide pre-blocked mapk3 antibody. The (A) input and (B, C) precipitates were detected by the same antibody.| Product Name | Mapk3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Mapk3 |
| Immunogen | synthetic peptide derived from the C-terminal region of zebrafish MAPK-3 protein |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IPWB |
| IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Zebra Fish |
| Concentration | 1mg/ml |
| Gene Synonyms | mitogen-activated protein kinase 3 fi06b09 wu:fi06b09 zERK1 mapk3 ERK1 |
|---|---|
| Target | Mapk3 |
Application
WB analysis of lysates from (A, C) zebrafish embryo (24 hpf) and (B, D) zebrafish larva (6 dpf) using Mapk3 antibody in the (A) absence or (B) presence of blocking peptide.
Application
IP analysis was perform with zebrafish embryonic (24-48 hpf) cytosolic lysate and either (B) Mapk3 antibody or (C) immunizing peptide pre-blocked mapk3 antibody. The (A) input and (B, C) precipitates were detected by the same antibody.| Application Notes | IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C for up to 24 months upon receiving the product. |
| Storage Buffer | MOPS buffer (pH 7.5) containing 0.1% BSA, 0.05% NaN3 and 50% glycerol |
