Application
Sample (50 ug of whole cell lysate) A: mouse spleen 10% SDS PAGE diluted at 1:10000Application
Sample(30 ug whole cell lysate) A:H1299 B:HeLa S3 10% SDS PAGE diluted at 1:3000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MAPKAP Kinase 3 antibody at 1:200 dilution.| Product Name | MAPKAP Kinase 3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MAPKAP Kinase 3 (mitogen-activated protein kinase-activated protein kinase 3)This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MAPKAP Kinase 3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Pig, Chicken, Rhesus Monkey, BovineRat (96%), Zebrafish (85%), Pig (98%), Chicken (93%), Rhesus Monkey (100%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 3PK antibody MAPKAP3 antibody MAPKAPK3 antibody MAPKAP kinase 3 antibody MAP kinase-activated protein kinase 3 antibody MAPK-activated protein kinase 3 antibody chromosome 3p kinase antibody mitogen-activated protein kinase-activated protein kinase |
|---|---|
| Molecular Weight(MW) | 43 kDa (note) (3) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
Sample (50 ug of whole cell lysate) A: mouse spleen 10% SDS PAGE diluted at 1:10000
Application
Sample(30 ug whole cell lysate) A:H1299 B:HeLa S3 10% SDS PAGE diluted at 1:3000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MAPKAP Kinase 3 antibody at 1:200 dilution.| Positive Control | H1299 , HeLaS3 , mouse spleen |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
