Application
Immunofluorescence staining of methanol-fixed Hela cells using MAPKAPK-2 (Phospho-Thr334) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MAPKAPK-2 (Phospho-Thr334) Antibody (left) or the same antibody preincubated with blocking peptide(right).Application
Western blot analysis of extracts from Hela cells untreated (lane 1) or treated with UV (lane 2) using MAPKAPK-2 (Phospho-Thr334) Antibody| Product Name | MAPKAPK-2 (phospho Thr334) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MAPKAPK-2 (Phospho Thr334)MAPKAPK-2 encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. |
| Immunogen | Peptide sequence around phosphorylation site of threonine 334 (P-Q-T(p)-P-L) derived from Human MAPKAPK-2. |
| Modification | p-Thr334 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MK2 MAPKAPK2 MAPK-activated protein kinase 2 MAPKAP kinase 2 MAP kinase-activated protein kinase 2 mitogen-activated protein kinase-activated protein kinase 2 |
|---|---|
| Target | MAPKAPK-2 (Phospho Thr334) |
| Molecular Weight(MW) | 49 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using MAPKAPK-2 (Phospho-Thr334) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MAPKAPK-2 (Phospho-Thr334) Antibody (left) or the same antibody preincubated with blocking peptide(right).
Application
Western blot analysis of extracts from Hela cells untreated (lane 1) or treated with UV (lane 2) using MAPKAPK-2 (Phospho-Thr334) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
