WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-MAPKAPK-2 (Phospho Thr334) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.| Product Name | MAPKAPK-2 (Phospho Thr334) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Modification | p-Thr334 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MAPKAPK2 |
|---|---|
| Gene Synonyms | MK2 MK-2 MAPKAP-K2 |
| Gene Full Name | MAPK activated protein kinase 2 |
| Gene Summary | This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names |
MAPKAPK2 MAP kinase-activated protein kinase 2 MAPK-activated protein kinase 2 MAPKAP kinase 2 MAPKAP-K2 MAPKAPK-2 MK-2 MK2 |
| Molecular Weight(MW) | 46 kDa(Observed band size: 49 kDa) |
| Cellular Localization | Cytoplasm . Nucleus . Phosphorylation and subsequent activation releases the autoinhibitory helix , resulting in the export from the nucleus into the cytoplasm. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-MAPKAPK-2 (Phospho Thr334) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.| Application Notes | WB:1:2000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
