Application
Sample (30 ug of whole cell lysate) A: HepG2 membrane 12% SDS PAGE diluted at 1:5000| Product Name | MARCH2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MARCH2 (membrane-associated ring finger (C3HC4) 2)MARCH2 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH2 reduces surface accumulation of several glycoproteins and appears to regulate early endosome-to-trans-Golgi network (TGN) trafficking (Bartee et al., 2004 [PubMed 14722266]; Nakamura et al., 2005 [PubMed 15689499]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MARCH2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Pig, BovineMouse (94%), Rat (94%), Zebrafish (81%), Xenopus laevis (80%), Pig (95%), Bovine (91%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MARCH-II antibody RNF172 antibody MARCH2 antibody membrane-associated RING finger protein 2 antibody E3 ubiquitin-protein ligase MARCH2 antibody RING finger protein 172 antibody membrane-associated RING-CH protein II antibody membrane-associated rin |
|---|---|
| Molecular Weight(MW) | 27 kDa (note) (3) |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein , Lysosome membrane; Multi-pass membrane protein , Endosome membrane; Multi-pass membrane protein |
Application
Sample (30 ug of whole cell lysate) A: HepG2 membrane 12% SDS PAGE diluted at 1:5000| Positive Control | HepG2 membrane |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
