Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using 39145 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Sample (20 µg of whole cell lysate) A: HeLa B: HeLa membrane 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | MARCH5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MARCH5 (membrane-associated ring finger (C3HC4) 5)MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MARCH5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Chicken, Rhesus Monkey, BovineMouse (100%), Rat (100%), Zebrafish (99%), Xenopus laevis (98%), Chicken (99%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ20445 antibody MARCH-V antibody MITOL antibody RNF153 antibody MARCH5 antibody membrane-associated RING-CH protein V antibody E3 ubiquitin-protein ligase MARCH5 antibody ring finger protein 153 antibody mitochondrial ubiquitin ligase antibody m |
|---|---|
| Molecular Weight(MW) | 31 kDa (note) (2) |
| Cellular Localization | Mitochondrion outer membrane; Multi-pass membrane protein |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using 39145 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Sample (20 µg of whole cell lysate) A: HeLa B: HeLa membrane 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HeLa , HeLa MF |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
