Application
Western blot analysis of KLF8 using KLF8 antibody. Human kidney tissue lysate in the 1) absence and 2) presence of immunizing peptide and 3) HEK293 lysate probed with 2 ug/ml of KLF8 antibody. Goat anti-rabbit Ig HRP secondary antibody and ECL substrate solution were used for this test.| Product Name | MARCKS (phospho Ser152/Ser153) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MARCKS ( phospho S152 + S153 )Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation. Moreover, MARCKS is a member of a family of calmodulin binding proteins. Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin. |
| Immunogen | This antibody was developed against synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser152 and phospho Ser 153 of MARCKS (Rat). |
| Modification | p-Ser152/Ser153 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | KINC Macs MARCKS (phospho S152/S153) MARCKS (phospho Ser152/Ser153) MARCKS (pS152/pS153) MARCKS (pSer152/pSer153) MARCKS phospho S152/S153 MARCKS phospho Ser152/Ser153 Phospho MARCKS (S152/S153) Phospho MARCKS (Ser152/Ser153) Phospho MARCKS (pS1 |
|---|---|
| Target | MARCKS |
| Cellular Localization | Plasma membrane |
Application
Western blot analysis of KLF8 using KLF8 antibody. Human kidney tissue lysate in the 1) absence and 2) presence of immunizing peptide and 3) HEK293 lysate probed with 2 ug/ml of KLF8 antibody. Goat anti-rabbit Ig HRP secondary antibody and ECL substrate solution were used for this test.| Positive Control | Rat brain |
|---|---|
| Application Notes | WB: Use at a dilution of 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: NoneConstituents: 50% Glycerol, 100µg/ml BSA, 150mM Sodium Chloride, 10mM HEPES. pH 7.5 |
