Application
Immunofluorescence staining of methanol-fixed Hela cells using MARCKS (Phospho-Ser158) AntibodyApplication
Western blot analysis of extracts from 3T3 cells using MARCKS(Phospho-Ser158) Antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).| Product Name | MARCKS (phospho Ser158) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MARCKS (Phospho Ser158)MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. |
| Immunogen | Peptide sequence around phosphorylation site of serine 158 (R-F-S(p)-F-K) derived from Human MARCKS. |
| Modification | p-Ser158 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 80K-L FLJ14368 FLJ90045 MACS PKCSL PRKCSL MARCKS phosphomyristin protein kinase C substrate 80 kDa protein light chain myristoylated alanine-rich C-kinase substrate myristoylated alanine-rich protein kinase C substrate (MARCKS 80K-L) myristoy |
|---|---|
| Target | MARCKS (Phospho Ser158) |
| Molecular Weight(MW) | 80 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using MARCKS (Phospho-Ser158) Antibody
Application
Western blot analysis of extracts from 3T3 cells using MARCKS(Phospho-Ser158) Antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
