Application
Western blot analysis of MARCKS (pS163) expression in MCF7 PMA-treated (A); SP2/0 PMA-treated (B); PC12 PMA-treated (C) whole cell lysates.| Product Name | MARCKS (phospho Ser163) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MARCKS (phospho Ser163)The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MARCKS. The exact sequence is proprietary. |
| Modification | p-Ser163 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PRKCSL 80K-L myristoylated alanine-rich protein kinase C substrate PKCSL MARCKS MACS |
|---|---|
| Target | MARCKS (phospho Ser163) |
| Tissue Specificity | Recognizes endogenous levels of MARCKS (pS163) protein. |
Application
Western blot analysis of MARCKS (pS163) expression in MCF7 PMA-treated (A); SP2/0 PMA-treated (B); PC12 PMA-treated (C) whole cell lysates.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
