Application
Cell lysates of HeLa(20?g) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Maspin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Application
Immunofluorescence of human HeLa cells stained with PI (Red) and monoclonal anti-Maspin antibody (1:500) with Alexa 488 (Green).| Product Name | Maspin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [AT2N6] to MaspinSerine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 5, also known as Maspin, is a tumor suppressor protein of serine proteinase inhibitor family. It plays an essential role in embryonic development through critical roles in cell adhesion. Also, it is present in normal breast and prostatic epithelial cells but downregulated in the respective carcinomas. |
| Immunogen | Recombinant Maspin (1-375aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | AT2N6 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| Western Blot: Use at 1:1000 ~ 2000. Recommended starting dilution is 1:1000.Optimal working concentrations should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Spi7. Protease inhibitor 5 Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 5 Serine (or cysteine) peptidase inhibitor SERPINB5 PI5 Serpin peptidase inhibitor Ovalbumin member5 Spi5 Serine (or cysteine) proteinase inhibitor cl |
|---|---|
| Target | Maspin |
| Light Chain Type | kappa |
Application
Cell lysates of HeLa(20?g) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Maspin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Application
Immunofluorescence of human HeLa cells stained with PI (Red) and monoclonal anti-Maspin antibody (1:500) with Alexa 488 (Green).| Application Notes | Western Blot: Use at 1:1000 ~ 2000. Recommended starting dilution is 1:1000.Optimal working concentrations should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide |
