Application
Immunoprecipitation of MATH1 protein from HepG2 whole cell extracts using 5 μg of MATH1 antibody [C2C3], C-term . Western blot analysis was performed using MATH1 antibody [C2C3], C-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MATH1 antibody at 1:200 dilution.Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
MATH1 antibody detects MATH1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with MATH1 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | MATH1 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MATH1 (protein atonal homolog 1)This protein belongs to the basic helix-loop-helix (BHLH) family of transcription factors. It activates E-box dependent transcription along with E47. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MATH1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Chimpanzee, BovineRat (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ATH1 antibody HATH1 antibody MATH-1 antibody bHLHa14 antibody ATOH1 antibody protein atonal homolog 1 antibody class A basic helix-loop-helix protein 14 antibody helix-loop-helix protein hATH-1 antibody atonal homolog 1 (Drosophila) antibody |
|---|---|
| Molecular Weight(MW) | 38 kDa (note) |
| Cellular Localization | Nucleus |
Application
Immunoprecipitation of MATH1 protein from HepG2 whole cell extracts using 5 μg of MATH1 antibody [C2C3], C-term . Western blot analysis was performed using MATH1 antibody [C2C3], C-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MATH1 antibody at 1:200 dilution.
Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
MATH1 antibody detects MATH1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with MATH1 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , HepG2 , NIH-3T3 , JC , BCL-1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
