Application
MAX antibody immunoprecipitates MAX protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. Control with 2 μg of preimmune rabbit IgG B. Immunoprecipitation of MAX protein by 2 μg of MAX antibody 15% SDS-PAGE The immunoprecipitated MAX protein was detected by MAX antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MAX antibody diluted at 1:500.| Product Name | MAX antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MAX (MYC associated factor X)The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Multiple alternatively spliced transcript variants have been described for this gene but the full-length nature for some of them is unknown. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MAX. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Cat, Rhesus Monkey, BovineMouse (99%), Rat (99%), Zebrafish (82%), Cat (100%), Rhesus Monkey (100%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC10775 antibody MGC11225 antibody MGC18164 antibody MGC34679 antibody MGC36767 antibody bHLHd4 antibody bHLHd5 antibody bHLHd6 antibody bHLHd7 antibody bHLHd8 antibody orf1 antibody MAX antibody class D basic helix-loop-helix protein 4 antibo |
|---|---|
| Molecular Weight(MW) | 18 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
MAX antibody immunoprecipitates MAX protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. Control with 2 μg of preimmune rabbit IgG B. Immunoprecipitation of MAX protein by 2 μg of MAX antibody 15% SDS-PAGE The immunoprecipitated MAX protein was detected by MAX antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MAX antibody diluted at 1:500.| Positive Control | U87-MG , SK-N-SH , IMR32 , SK-N-AS |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
